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Biol Bull 100: 1-14. (February 1951)
© 1951 Marine Biological Laboratory
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THE ACTION OF SAPONIN AND ITS NON-HEMOLYTIC MODIFICATION ON GROWTH

JOSEPH M. BUTROS 1

1 Biology Department, American University of Beirut. Beirut, Lebanese Republic

1. Onion root elongation was arrested within two days by 0.1 per cent Merck saponin and 0.3 per cent Baker saponin.

2. No mitotic figures were discerned in these cells; the nuclei of all cells were in the resting stage.

3. Lower concentrations, down to a certain point, reduced the rate of growth of the roots. Below that the very dilute saponins appeared to be growth stimulating.

4. A method of preparing a non-hemolytic suspension of saponin cholesteride is given.

5. The properties of non-hemolytic saponin cholesteride are described. Its toxicity to animals is much less than saponin; also it arrests onion root growth. It also retards the development of tadpoles. Its cytolytic power to mammalian tissues does not seem to have changed.

6. A non-hemolytic preparation of saponin-eosin is found to he partially inhibiting to onion root growth.

7. The possible explanations for this action are discussed.






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Copyright © 1951 by the Marine Biological Laboratory.