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1 Zoological Laboratory, State University of Iowa, Iowa City
1. The endogenous oxygen uptake of the homogenate of the embryo of the grasshopper, Melanoplus differentialis, along with the intracellular constituents of the embryonic cells has been measured.
2. The endogenous oxygen uptake of intact washed nuclei and their homogenate as well as that of the intranuclear constituents has also been measured.
3. Respiratory enzymes of the nucleus are located in the solid particles obtained from fractional centrifugation.
4. Enzymes of cytoplasm and nucleus appear specific since no change in oxygen uptake occurs when they are added to reciprocal substrates.
5. Fractions of homogenates of embryo or nuclei when recombined have oxygen uptakes of approximately 90-95 per cent of the original homogenates.
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