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1 Philadelphia Aquarium Society, Philadelphia, Pennsylvania
1. Evidence is presented to indicate an as yet ill-defined cyclical production of ova in virgin female Lebistes.
2. Copulation occurs within 12-24 hours after virgin female fishes are placed in aquaria with two male fishes. Sperm migration in the female gonaduct and amphimixis occurs rapidly after mating.
3. The time of fertilization of ova in relation to a previous brood has been determined by the use of sperm replacement studies with genetic mutation tracers.
4. Evidence is presented to indicate a cyclical nature of maximum and minimum brood production of individual female fish maintained under constant conditions of temperature and light.
5. The possible factors affecting brood production are discussed.
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