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1 Departments of Zoology, Barnard College and Columbia University, New York, N. Y.
1. In an effort to dissociate the factors responsible for the failure of development in Rana pipiens
x Rana sylvatica
hybrids, a study has been made of the development of haploid embryos composed of pipiens cytoplasm and sylvatica chromosomes. These androgenetic hybrids develop only to the late blastula stage.
2. The competence of the presumptive ectoderm of these haploid hybrids was tested by transplantation to neurulae of Rana palustris. The results indicated a total lack of competence.
3. Aside from the main problem with which the paper is concerned, it was found that the various classes of embryos used could be arranged in order of decreasing competence of the presumptive ectoderm. The sequence was as follows: diploid pipiens, haploid pipiens, pipiens
x sylvatica
diploid hybrids and (pipiens
) x sylvatica
haploid hybrids.
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