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1 Departments of Zoology, Barnard College, Columbia University New York 27, New York
1. A proteolytic enzyme was demonstrated, by use of a new microanalytical technique, to be present in the endostyle of the ammocoetes larva of Petromyzon marinus.
2. The protease has an optimum pH of 4.0. The hydrolysis of hemoglobin (expressed as micrograms of tyrosine released) under the conditions employed, and within a period of three hours, is a straight line function of time. The hydrolytic activity of the endostylar enzyme is also a straight line function of enzymatic concentration, but within restricted and defined limits.
3. The probable site of action of the hydrolysis of the endostylar thyroprotein is discussed in regard to the usefulness of the investigated endostylar protease.
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