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Biol Bull 112: 21-27. (February 1957)
© 1957 Marine Biological Laboratory
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EVIDENCE FROM SEA URCHIN-SAND DOLLAR HYBRID EMBRYOS FOR A NUCLEAR CONTROL OF ALKALINE PHOSPHATASE ACTIVITY

REED A. FLICKINGER 1

1 Dept. of Zoology, University of California, Los Angeles, California and Friday Harbor Laboratories, University of Washington, Friday Harbor, Washington

1. Alkaline phosphatase estimations upon sea urchin embryos (S. purpuratus) showed a slow rate of increasing activity up to the pluteus stage while sand dollar embryos (Dendraster excentricus) showed a very rapid increase up to this same stage.

2. Hybridization between Strongylocentrotus purpuratus female and Dendraster excentricus male resulted in alkaline phosphatase activity at the prism stage which was intermediate between that of the two homologous species and higher than that of the maternal species. This is interpreted as indicating a nuclear control of alkaline phosphatase activity.

3. Addition of substrate (0.1 M sodium beta glycerophosphate) to cultures of developing S. purpuratus embryos did not affect an increase in acid or alkaline phosphatase activity.







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Copyright © 1957 by the Marine Biological Laboratory.