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Biol Bull 113: 414-425. (December 1957)
© 1957 Marine Biological Laboratory
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STUDIES ON THE ISOLATED ISLET TISSUE OF FISH. II. THE EFFECT OF ELECTROLYTES AND OTHER FACTORS ON THE OXYGEN UPTAKE OF PANCREATIC ISLET SLICES OF TOADFISH, USING THE CARTESIAN DIVER MICRORESPIROMETER

ARNOLD LAZAROW 1, S. J. COOPERSTEIN 1, D. K. BLOOMFIELD 1, and C. T. FRIZ 1

1 Departments of Anatomy, University of Minnesota, Minneapolis 14, Minnesota; Western Reserve University, Cleveland, Ohio; and the Marine Biological Laboratory, Woods Hole, Mass.

1. The metabolic activity of toadfish islet slices was measured in a cartesian diver microrespirometer under varying experimental conditions. The effects of pH, specific electrolytes, tonicity, trace metals, and protein addition were studied.

2. The metabolic activity was not affected by varying the pH of the medium between 6.2 and 8.0. The addition of phosphate ion stimulated the respiration. The maximum stimulation was observed when the external medium contained 0.066 M phosphate.

3. The respiration of islet slices was increased when the tonicity of the suspending media was reduced; optimal respiration was observed in a hypotonic medium with a tonicity equivalent to 0.075 M NaCl; this corresponds to a phosphate buffer concentration of 0.054 M. This is slightly sub-optimal with respect to phosphate ion concentration. Since the metabolic activity of islet tissue slices suspended in 0.054 M phosphate buffer (optimal tonicity but sub-optimal phosphate) is slightly greater than in 0.066 M phosphate buffer (optimal phosphate but sub-optimal tonicity), subsequent studies were carried out using 0.054 M phosphate buffer.

4. High concentrations of calcium (0.001-0.002 M) inhibited the respiration of islet slices. The addition of serum protein, lower concentrations of calcium ion (0.0005 M), magnesium ion (0.0002-0.01 M), and small amounts of trace metals (boron, molybdenum, iron, manganese, zinc) did not stimulate the respiration of islet tissue slices.




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D. Watkins, S. J. Cooperstein, P. K. Dixit, and A. Lazarow
Insulin Secretion from Toadfish Islet Tissue Stimulated by Pyridine Nucleotides
Science, October 11, 1968; 162(3850): 283 - 284.
[Abstract] [PDF]


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Copyright © 1957 by the Marine Biological Laboratory.