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Biol Bull 115: 74-80. (August 1958)
© 1958 Marine Biological Laboratory
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DISPERSAL OF THE GELATINOUS COAT MATERIAL OF MELLITA QUINQUIESPERFORATA EGGS BY HOMOLOGOUS SPERM AND SPERM EXTRACTS

JOHN W. BROOKBANK 1

1 Department of Biology, University of Florida, Gainesville, Florida

1. A factor causing the dispersal of the gelatinous coat of Mellita eggs was shown to be present on the surface of Mellita sperm and in frozen-thawed extracts of sperm suspensions.

2. The factor was separable from antifertilizin on the basis of temperature and pH sensitivity.

3. The factor did not degrade fertilizin in solution, but released this substance from the gel surrounding the egg.

4. Active extracts were capable of dissolving fertilizin-antifertilizin precipitation membranes, formed on the surface of the fertilizin coat of unfertilized eggs in the presence of Arbacia or Mellita antifertilizin.




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J. Green and R. Summers
Ultrastructural demonstration of trypsin-like protease in acrosomes of sea urchin sperm
Science, July 18, 1980; 209(4454): 398 - 400.
[Abstract] [PDF]


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