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Biol Bull 117: 518-545. (December 1959)
© 1959 Marine Biological Laboratory
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STUDIES OF EARLY CLEAVAGE IN THE SURF CLAM, SPISULA SOLIDISSIMA, USING METHYLENE BLUE AND TOLUIDINE BLUE AS VITAL STAINS

LIONEL I. REBHUN 1

1 Department of Biology, Princeton University, Princeton, New Jersey; and Marine Biological Laboratory, Woods Hole, Massachusetts

1. Techniques for vitally staining oocytes of Spisula solidissima with certain basic dyes are described.

2. Such staining reveals small particles in the cytoplasm of the egg.

3. After fertilization the particles show very specific movements and localization changes which can be described by saying that the particles follow the centrioles through cleavage.

4. Spindle movements and position changes can easily be followed and are described in detail.

5. The results are discussed in the light of related work in the literature.






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Copyright © 1959 by the Marine Biological Laboratory.