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Biol Bull 118: 70-78. (February 1960)
© 1960 Marine Biological Laboratory
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SURFACE ANTIGEN DYNAMICS IN THE SLIME MOLD, DICTYOSTELIUM DISCOIDEUM

JAMES H. GREGG 1

1 Department of Biology, University of Florida, Gainesville, Florida, and Marine Biological Laboratory, Woods Hole, Mass.

1. Methods are described whereby antigenic material of the slime mold, D. discoideum, effective in antibody production in rabbits, is prepared.

2. Antisera produced in response to migrating pseudoplasmodia, mature spores and mature spore surface antigens effected maximal agglutination of amoebae. Such antisera effected only minimal agglutination of spores.

3. Antisera of the above types absorbed by amoebae and spores in all combinations revealed the presence of a surface antigen(s) on the spores which is (are) not common to amoebae from early aggregates or migrating pseudoplasmodia.

4. It has been suggested that a relationship exists between the adhesive properties of the amoebae and spores and their agglutinating properties in the presence of antisera.






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