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Biol Bull 123: 295-301. (October 1962)
© 1962 Marine Biological Laboratory
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FREE GLYCEROL IN DORMANT CYSTS OF THE BRINE SHRIMP ARTEMIA SALINA, AND ITS DISAPPEARANCE DURING DEVELOPMENT

JAMES S. CLEGG 1

1 Department of Biology, The Johns Hopkins University, Baltimore 18, Maryland

1. Free glycerol was identified as a major carbohydrate component of the dormant cysts of Artemia salina.

2. The amount of glycerol present in cysts aged for a year in the dry state was found to be about 5% of the cyst weight, and was shown to be restricted to the embryonic part of the cyst.

3. Glycerol content decreased slightly during the formation of the nauplius and then rapidly decreased to a very low level after the nauplius emerged from the cyst. The decrease in glycerol content could not account for the synthesis of glycogen during formation of the nauplius.

4. The glycerol, trehalose, and glycogen contents, and the viability of cysts aged up to 28 years were determined.






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