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Biol Bull 125: 61-68. (August 1963)
© 1963 Marine Biological Laboratory
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EFFECT OF THIOUREA ON MOULTING AND PUPATION OF THE SILKWORM, BOMBYX MORI L

WANDA CHMURZYNacuteSKA 1 and LECH WOJTCZAK 1

1 Department of Biochemistry, Nencki Institute of Experimental Biology, Warsaw, Poland

1. Thiourea injected into silkworm larvae, Bombyx mori L., was found to inhibit the activity of phenoloxidase.

2. The effect of thiourea injection on moulting and pupation of the larvae was examined. When the injection preceded 24 hours or less the fourth moulting period or the spinning, disturbances in larval nioulting and in pupation were observed, respectively. They consisted of a retention of the old cuticle and an accumulation of a haemolymph-like fluid between the old and the new cuticles. Histological exaniination revealed a partial inhibition and a delay of the sclerotization of pupal cuticle.






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