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Biol Bull 126: 1-7. (February 1964)
© 1964 Marine Biological Laboratory
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THE EFFECT OF ANTISERA AGAINST FERTILIZIN ON THE UPTAKE OF ORTHOPHOSPHATE BY SEA URCHIN EGGS

JOHN W. BROOKBANK 1

1 Department of Microbiology, College of Medicine, and the Department of Biology University of Florida, Gainesville, Florida

1. Normal rabbit sera and antisera prepared in rabbits against sea urchin fertilizin were shown to have an inhibitory effect on uptake of phosphate by fertilized eggs. Immune sera caused more pronounced decreases in phosphate transport.

2. Bovine serum albumin solutions caused similar decreases rate of phosphate transport.

3. Inhibitory effects of proteins were presumed to be due to a combination of protein with the egg surface, rather than a binding of phosphate by proteins in solution.

4. Antisera against fertilizin were without effect on phosphate uptake by unfertilized eggs.

5. The results are discussed in light of other works on egg activation and phosphate uptake.






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Copyright © 1964 by the Marine Biological Laboratory.