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Biol Bull 126: 253-263. (April 1964)
© 1964 Marine Biological Laboratory
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AN AIR-ABRASIVE METHOD FOR THE MICRODISSECTION OF MINERALIZED TISSUES

ULF A. FRIBERG 1, PHILIP T. LEVINE 1, and MELVIN J. GLIMCHER 1

1 Department of Orthopedic Surgery, Harvard Medical School and The Massachusetts General Hospital, and the Forsyth Dental Center, Boston 14, Massachusetts

A method for the microdissection of mineralized tissues is described, utilizing high speed, gas-impelled abrasive for the sectioning. The method was developed to make possible the preparation of histologically defined samples of such tissues for biochemical and biophysical analysis. Mineralized tissue sections are mounted on a motorized, cross-feed stage and passed below a fixed nozzle delivering the abrasive stream. The operator controls the direction and progress of the microdissection through a stereo microscope. This procedure may be readily adapted to a wide variety of preparatory tasks involved in the study of mineralized tissues.






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Copyright © 1964 by the Marine Biological Laboratory.