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Biol Bull 129: 72-78. (August 1965)
© 1965 Marine Biological Laboratory
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THE INDUCTIVE ROLE OF THE YOLK EPITHELIUM IN THE DEVELOPMENT OF THE SQUID, LOLIGO PEALII (LESUEUR)

JOHN M. ARNOLD 1

1 Marine Biological Laboratory, Woods Hole, Massachusetts 02543, and the Department of Zoology, University of Minnesota, Minneapolis, Minnesota 55455

1. Techniques for the in vitro culture of dechorionated Loiigo pealii embryos, involving the use of whole adult squid blood, sterile sea water, and antibiotics, have been devised. Essentially normal development will take place in this culture medium.

2. The embryos in stages 16 and 17 are composed essentially of three components: an outer layer of cells, an inner cellular yolk epithelium, and the central mass of yolk. When the outer layer of cells and the yolk epithelium are isolated together, normal histogenesis and development will occur. When the outer layer of cells is isolated by itself, no differentiation occurs. This leads to the conclusion that the yolk epithelium induces the outer layer of cells to differentiate. This conclusion was upheld by grafting and deletion experiments.

3. The role of the yolk epithelium, therefore, may be in acting as a "morphogenetic inductive map."






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