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1 Marine Biological Laboratory, Woods Hole, Massachusetts 02543, and Department of Biology, Rice University, Houston, Texas 77001
1. The excystment of C. lingua metacercariae is mediated by the action of digestive enzymes. The same sequence of enzyme solutions as that met with in the host's digestive tract is required.
2. Reducing and surface-active agents, and a carbon dioxide atmosphere enhance excystment.
3. None of these factors proved to be an absolute requirement, but their combined effect was to stimulate excystment within a biologically useful time period.
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