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1 Marine Biological Laboratory, Woods Hole, Massachusetts 02543
1. The aging of semen was investigated in terms of sperm motility. A method for rating motility was devised. It was based on the observation that motile sperm sedimented faster than formalin-killed controls when subjected to low-speed centrifugation.
2. The aging of semen was found to involve the loss of motility, the loss of fertilizing capacity and disappearance of sperm from suspension. All three of these factors declined most rapidly at first and more slowly later on. The rapid loss of fertilizing capacity and the disappearance of sperm could be delayed by suppressing motility with hydrogen ions or carbon dioxide.
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