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1 Biology Department, San Diego State College, San Diego, California
1. The spawning of Ciona intestinalis with respect to light was studied, using both white light and monochromatic light.
2. A one-hour dark-adaption period followed by exposure to light resulted in spawning by 66.6% of the 884 animals tested.
3. Spawning occurs an average of 27.3 minutes after the onset of illumination.
4. Illumination need not be continuous until spawning occurs; the animals spawn when returned to the dark after a short illumination period, provided they have received enough energy.
5. The action spectrum for spawning suggests cytochrome c as a chromophore.
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