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Biol Bull 132: 229-243. (April 1967)
© 1967 Marine Biological Laboratory
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GENETIC AND DEVELOPMENTAL STUDIES ON BOTRYLLUS SCHLOSSERI

ROGER MILKMAN 1

1 Marine Biological Laboratory, Woods Hole, Massachusetts 02543 and Department of Zoology, Syracuse University, Syracuse, New York 13210

1. Properties of Botryllus schlosseri which give it outstanding promise for studies in developmental genetics are reviewed.

2. Laboratory culture procedures, in vitro fertilization, and a method for raising embryos in vitro are described. Controlled successions of complete life cycles can now be achieved in any laboratory.

3. Experiments involving colony fusion, subsequent vascular budding, and the analysis of color patterns in resultant systems suggest that cells of the simple vessel walls govern the morphology of the regenerated zooids.

4. Results of some preliminary genetic crosses are reported.




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