THE EFFECT OF TRYPSIN ON REGENERATION INHIBITORS IN TUBULARIA

¹ Department of Anatomy, Tulane University Medical School, New Orleans, Louisiana

1. Supernatant of homogenate obtained from regenerating Tubularia stems was electrophoresed through starch gel.

2. Starch cores from the gel were partially inserted into the coelenterons of stems in an earlier stage of reconstitution.

3. These stems were then oriented in an electric current so that any charged material in the cores would migrate toward the stems.

4. The electrophoresed supernatant from primordial homogenate retarded the rate of reconstitution.

5. The addition of a small amount of trypsin prior to electrophoresis removed the regeneration-inhibiting effect of the supernatant, thus indicating a polypeptide or protein nature for the inhibiting substance(s).