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Biol Bull 138: 379-388. (June 1970)
© 1970 Marine Biological Laboratory
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THE BLOOD CELLS AND TUNIC OF THE ASCIDIAN HALOCYNTHIA AURANTIUM (PALLAS). II. HISTOCHEMISTRY OF THE BLOOD CELLS AND TUNIC

MICHAEL J. SMITH 1

1 Department of Zoology, University of British Columbia, Vancouver 8, Canada

1. The similar staining properties of the vacuolar blood cells of Halocynthia aurantium indicate the relationship of the cell types constituting this group.

2. The mature morula cells and dispersed vesicular cells of Halocynthia do not stain for the presence of polysaccharides, but do stain for protein.

3. Changes in the staining properties of mature morula and dispersed vesicular cells between blood and tunic indicate that these cells contribute protein to tunic structures.

4. The tunic matrix and epidermis show stain affinities characteristic of acid mucopolysaccharide material.

5. The tunic matrix fibers display a consistent negative reaction to the P.A.S. technique. Neither enzymatic nor chemical treatment of tunic increases the reactivity of the fiber to P.A.S. reagent.






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Copyright © 1970 by the Marine Biological Laboratory.