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Biol Bull 139: 296-303. (October 1970)
© 1970 Marine Biological Laboratory
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REVERSIBLE RESPONSE TO PUROMYCIN AND SOME CHARACTERISTICS OF THE UPTAKE AND USE OF AMINO ACIDS BY UNFERTILIZED SEA URCHIN EGGS

F. ROY MACKINTOSH 1 and EUGENE BELL 2

1 Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139
2 Marine Biological Laboratory, Woods Hole, Massachusetts 02543

Protein synthesis in embryos and oocytes is irreversibly inhibited when the cells are returned to puromycin-free sea water after previous exposure to puromycin. In unfertilized eggs, on the other hand, it is not irreversibly inhibited. This effect and others are used to show that mature unfertilized eggs are actively engaged in protein synthesis, and that direct assessment of the relative rate of protein synthesis in mature eggs is not hindered by contamination with a small population of very active cells.






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Copyright © 1970 by the Marine Biological Laboratory.