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Biol Bull 139: 321-332. (October 1970)
© 1970 Marine Biological Laboratory
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A GENERAL METHOD FOR THE MONOXENIC CULTIVATION OF THE DAPHNIDAE

JAMES S. MURPHY 1

1 The Rockefeller University, New York, New York 10021

Fourteen species of the family Daphnidae have been established under continuous monoxenic cultivation utilizing Chlamydomonas reinhardii as sole food organism in a medium consisting of calcium acetate, antibiotics, albumin, trace elements and the water soluble vitamins, folic acid, B12, calcium pantothenate, choline, pyridoxal, inositol, thiamin, nicotinamide, riboflavin, biotin and putrescine. The Daphnidae under cultivation include Daphnia magna, D. pulex, D. galeata mendotae, D. laevis, D. dubia, D. retrocurva, D. parvula, D. ambigua, D. catawba, Moina macrocopa Scapholeberis mucronata, Simocephalus serrulatus, Ceriodaphnia reticulata, and C. quadrangula. The requirements for vitamins for some species are more complex than for others. The complete medium is superior for all but Scapholeberis mucronata and markedly increases the lifespan and fertility of Moina macrocopa.






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Copyright © 1970 by the Marine Biological Laboratory.