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1 Department of Zoology, University of Hawaii, Honolulu, Hawaii 96822
(1) A technique was developed to determine the number of sperm present for each fertilization, under standardized conditions. Gametes from six species of urchins were examined.
(2) The reliability of the method was examined by analyzing 16 repeated crosses between the same set of gametes. Variability was evaluated by crossing gametes from four males and four females in the 16 possible combinations simultaneously. The results from many such crosses were pooled for statistical analysis.
(3) A wide range of values was obtained for each species and the data were not normally distributed when all values were considered.
(4) There were probably differences between the average fertility of gametes from several of the species examined while other species produced gametes of about the same average fertility as those from other taxonomically closely related species.
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