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Biol Bull 146: 335-342. (June 1974)
© 1974 Marine Biological Laboratory
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INITIATION OF METAMORPHOSIS IN LABORATORY CULTURED SEA URCHINS

R. ANDREW CAMERON 1 and RALPH T. HINEGARDNER 2

1 Thimann Laboratories, University of California, Santa Cruz, California 95064
2 Marine Biological Laboratory, Woods Hole, Massachusetts 02543

A method is described for studying the initiation of metamorphosis in laboratory cultured sea urchins. Two stimuli are usually needed to initiate metamorphosis: a non-particulate organic chemical cue and a surface. The chemical probably has a molecular weight of less than 5,000 and is of bacterial origin. Electrical stimulation can be used to initiate metamorphosis of Arbacia punctulata larvae.




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