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Biol Bull 152: 406-414. (June 1977)
© 1977 Marine Biological Laboratory
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ISOLATION OF NEMATODE INHIBITOR FROM HEMOLYMPH OF THE SNAIL, HELIX ASPERSA

CHARIYA RATANARAT-BROCKELMAN 1

1 Laboratory of Parasitology, The Rockefeller University, New York, New York 10021

Hemolymph plasma of the snail Helix aspersa which inhibits maturation and reproduction of its mantle cavity-inhabiting nematode, Rhabditis maupasi, was separated biochemically for the active proteinaceous component.

Isolation of the active inhibitor was performed using ion-exchange chromatography in combination with subsequent gel filtration. The isolated peaks were bioassayed in vitro on nematode larvae. The fractions harboring inhibitory protein suppressed larval growth and adult reproduction in vitro.

The isolated fraction was purified by gel filtration and characterized on the basis of a single band on starch zone electrophoresis and positive reaction only with folin-phenol reagent.






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Copyright © 1977 by the Marine Biological Laboratory.