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Biol Bull 154: 47-54. (February 1978)
© 1978 Marine Biological Laboratory
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BIPHASIC PARTICULATE MEDIA FOR THE CULTURE OF FILTER-FEEDERS

D. E. CONKLIN 1 and L. PROVASOLI 2

1 The Bodega Marine Laboratory, University of California, Davis, P.O. Box 247, Bodega Bay, California 94923
2 Haskins Laboratories, Biology Department, Yale University, New Haven, Connecticut 06520

1. Over 200 parthenogenetic generations of the freshwater Cladocera Moina macrocopa were obtained aseptically in three artificial media.

2. The media have two phases: a liquid phase supplying mineral salts, water soluble vitamins, nucleic acids, and a liver extract and a fine particulate phase made from coagulated proteins, starch and lipid factors.

3. The particulate phase supplies the bulk nutrients very efficiently; hence, this type of media may be useful for growing other filter-feeders.






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Copyright © 1978 by the Marine Biological Laboratory.