Biol. Bull. Sign up for etocs!
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Biol Bull 155: 563-575. (December 1978)
© 1978 Marine Biological Laboratory
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by LAMBERT, D. T.
Right arrow Articles by FINGERMAN, M.
Right arrow Search for Related Content
PubMed
Right arrow Articles by LAMBERT, D. T.
Right arrow Articles by FINGERMAN, M.

COLCHICINE AND CYTOCHALASIN B: A FURTHER CHARACTERIZATION OF THEIR ACTIONS ON CRUSTACEAN CHROMATOPHORES USING THE IONOPHORE A23187 AND THIOL REAGENTS

DREW T. LAMBERT 1 and MILTON FINGERMAN 1

1 Department of Biology, Tulane University, New Orleans, Louisiana 70118

1. Using the prawn, Palaemonetes pugio, and the fiddler crab, Uca pugilator, the modes of action of cytochalasin B and colchicine in inhibiting pigment granule movement were further characterized.

2. Colchicine was unable to inhibit, in ovarian erythrophores of Palaemonetes pugio, pigment concentration caused by the ionophore A23187. It did, however, inhibit somewhat the pigment dispersion that normally occurs upon placing A23187-treated erythrophores in Ca++-free, EGTA-containing saline.

3. Inhibition by colchicine of pigment concentration in melanophores of Uca pugilator was not prevented by high concentrations of glutathione or cysteine hydrochloride. Pigment concentration was inhibited by mersalyl acid, and this was prevented by glutathione.

4. Cytochalasin B inhibited ionophore A23187-induced pigment concentration in ovarian erythrophores of Palaemonetes pugio.

5. Electron microscopy revealed a filamentous matrix interconnecting the pigment granules of Uca pugilator.

6. Speculation is presented as to the modes of action of cytochalasin B and colchicine, and a heuristic model for the control of pigment movement is put forward.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1978 by the Marine Biological Laboratory.