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Biol Bull 166: 178-188. (February 1984)
© 1984 Marine Biological Laboratory
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D-GALACTOSIDE SPECIFIC LECTINS FROM COELOMOCYTES OF THE ECHIURAN, URECHIS UNICINCTUS

TAEI MATSUI 1

1 Department of Biology, Faculty of Science, Nagoya University Chikusa, Nagoya 464, Japan

Two lectins specific for D-galactosides with molecular weights of 31,000 and 34,000 (estimated by SDS-PAGE, under reduced conditions) were purified from coelomocytes of the echiuran, Urechis unicinctus. They were eluted together from a BioGel P-100 column as a single peak with an apparent molecular weight of 35,000. They closely resembled each other in saccharide specificity, isoelectric point, and electrophoretic mobility under unreduced conditions. The hemagglutinating activity of both lectins was inhibited by a glycoconjugate fraction obtained from the Pronasedigest of coelomocytes.

Submitted on September 14, 1983
Accepted on November 23, 1983




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