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Biol Bull 166: 0-. (June 1984)
© 1984 Marine Biological Laboratory
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ERRATUM

THE BIOLOGICAL BULLETIN, Volume 165, Number 2, Page 514

The following correction should be made in the first abstract by A. Eisen, G. T. Reynolds, S. Wieland, and D. P. Kiehart entitled, "Calcium transients during fertilization in single sea urchin eggs," appearing on page 514. The title and authors are incorrect and the abstract should now read as follows:

Calcium transients during early development in single starfish (Asterias forbesi) oocytes and eggs. A. EISEN (Children's Hospital of Philadelphia) AND G. T. REYNOLDS.

Two events associated with a putative transient increase in cytoplasmic free calcium include: activation of the starfish oocyte with the maturation hormone 1-methyl adenine (1-MA), and fertilization of the starfish egg with sperm. These events were investigated in single oocytes and eggs by the detection of calcium specific luminescence from single cells injected with an acetylated form of the photoprotein aequorin (10 mg/ml in 10 mM HEPES, 0.2 mM EGTA, pH 7.0 tp 3% of cell volume). Using a microscope-photomultiplier and a microscope-image intensifler-SIT vidicon detector sensitive to <10-7 M Ca++ we found: 1) a barely detectable(le10-7 M) change in free calcium from oocytes in response to 1-MA (final concentration ca. 150 µM), and 2) a large (ca. 10-6) increase from eggs fertilized with sperm 15 minutes after application of 1-MA and 5 minutes after general vesicle breakdown (17°). The calcium-aequorin luminescence increases as it propagates over 30-40 s and decays uniformly over 200-300 s. The absence of a calcium transient in the Asterias forbesi differs significantly from the large (ca. 10-6 M) transient reported in the M. glacialis oocyte and is suggested as being a common feature of starfish oocyte activation. The calcium transient at fertilizationin Asterias eggs is similar to that described in several species of sea urchin (A. punctulata and L. variegazus) although the propagation time is much longer in the starfish egg.

We thank Dr. O. Shimomura for the gift of acetylated aequorin. We thank Dr. A. J. Walton for the use of his microscope objectives and assistance in the experiments. This work was supported by DOE Contract EY-76-S-02-3120 to G.T.R.






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