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Biol Bull 175: 185-192. (October 1988)
© 1988 Marine Biological Laboratory
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Characterization of the Molt Stages in Penaeus vannamei: Setogenesis and Hemolymph Levels of Total Protein, Ecdysteroids, and Glucose

SIU-MING CHAN 1, SUSAN M. RANKIN 1, and LARRY L. KEELEY 1

1 Laboratories for Invertebrate Neuroendocrine Research, Department of Entomology, Texas A&M University, Texas Agricultural Experimental Station, College Station, Texas 77843

The molting cycle of Penaeus vannamei juveniles was characterized by distinct and predictable changes in the setae of pleopods. The molt pattern was diecdysic with a relatively short intermolt period (40%) and a long proecdysial period (>53%). The levels of both total protein and ecdysteroids increased in the hemolymph during proecdysis, whereas the level of hemolymph glucose was low at metecdysis and proecdysis and maximal during anecdysis. As revealed by SDS-PAGE, the relative concentrations of two polypeptides (32 kD; 175 kD) changed during the molting cycle.

Submitted on March 10, 1988
Accepted on July 28, 1988






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