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Biol Bull 176: 8-13. (February 1989)
© 1989 Marine Biological Laboratory
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Characterization of a Factor with Oocyte Maturation Inducing Activity in Spisula

ARJUN L. KADAM 1 and S. S. KOIDE 2

1 Center for Biomedical Research, The Population Council, 1230 York Avenue, New York, New York 10021
2 Marine Biological Laboratory, Woods Hole, Massachusetts 02543

A substance that induces germinal vesicle breakdown (GVBD) of Spisula oocytes in vitro was isolated from Spisula body fluid (SBF) and ganglion cells by ultrafiltration through PM-lO membrane filter, charcoal extraction, and reversed phase HPLC. Its retention time on HPLC elution is coincident with that of reference serotonin (5-hydroxytryptamine, 5-HT) creatinine sulfate. The HPLC-purified substance from SBF and nerve ganglion induced GVBD in Spisula oocytes in a dose-dependent manner. 5-HT was identified in Spisula body fluid and ganglion cells by HPLC-electrochemical detection. The present results show that the factor in Spisula body fluid and ganglion cells that induces GVBD in Spisula oocytes in vitro is 5-HT or a closely related compound.

Submitted on March 3, 1988
Accepted on November 21, 1988




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I. Masseau, P. Bannon, M. Anctil, and F. Dube
Localization and Quantification of Gonad Serotonin During Gametogenesis of the Surf Clam, Spisula solidissima
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[Abstract] [Full Text] [PDF]


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