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Biol Bull 177: 344-349. (December 1989)
© 1989 Marine Biological Laboratory
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Cloned cDNA and Antibody for an Ovarian Cortical Granule Polypeptide of the Shrimp Penaeus vannamei

JAMES Y. BRADFIELD 1, ROBERT L. BERLIN 1, SUSAN M. RANKIN 1, and LARRY L. KEELEY 1

1 Laboratories for Invertebrate Neuroendocrine Research, Department of Entomology, Texas A&M University, College Station, Texas 77843-2475

A cloned cDNA was generated to a transcript for a major ovarian polypeptide (200 kDa) of the South American white shrimp, Penaeus vannamei. The cloned cDNA hybridized to a single transcript in ovaries but not to RNA from the hepatopancreas or muscle. For immunodetection and quantitation, a monospecific polyclonal antibody was raised against the cDNA translation product expressed in bacteria. The antibody was used to show that the 200 kDa ovarian polypeptide accumulated in cortical granules during ovarian development to comprise sim11% of the total ovarian protein and disappeared during early embryonic development. These studies begin to explain a gene-product relationship essential for reproduction in P. vannamei.

Submitted on March 23, 1989
Accepted on August 22, 1989




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