The Biological Bulletin, Vol 182, Issue 1 135-144, Copyright © 1992 by Marine Biological Laboratory

PHYSIOLOGY

Visual Cells and Pigments in a Demersal Fish, the Black Sea Bass (Centropristis striata)

K. V. Singarajah and F. I. Harosi
Laboratory of Sensory Physiology, Marine Biological Laboratory, Woods Hole, Massachusetts 02543

Using a single-beam, wavelength-scanning, dichroic microspectrophotometer, we measured absolute absorbance, bleaching difference absorbance, and linear dichroism spectra from isolated retinal receptors of the black sea bass, Centropristis striata. We determined, among other properties, the wavelength of peak {alpha}-band absorbance ({lambda}max) of the pigment of the receptor cells. Out of well over 100 recordings, we found only 3 spectral types of visual pigment. The shortest-wavelength-absorbing type ({lambda}max = 463 +/- 2 nm) was present only in single cones. Both members of the double cones contained the longest-wavelength-absorbing pigment of the three, with {lambda}max = 527 +/- 5 nm. Rods were found to bear a typical rhodopsin, with {lambda}max = 498 +/- 2 nm. Thus, the retina of this predatory demersal fish appears to use a set of three closely spaced visual pigments, with {lambda}max clustering about 500 +/- 30 nm. This remarkable feature is discussed in relation to photic conditions in the habitat.