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The Biological Bulletin, Vol 183, Issue 2 242-247, Copyright © 1992 by Marine Biological Laboratory

DEVELOPMENT AND REPRODUCTION

In vivo Incorporation of Labeled Methionine into Proteins, Vitellogenin, and Vitellin in Females of the Penaeid Shrimp Penaeus semisulcatus de Haan

S. Shafir, M. Ovadia and M. Tom
Israel Oceanographic and Limnological Research, P.O. Box 8030, Haifa, 31080, Israel

[35S]-Methionine was injected into 11 intact vitellogenic females of the penaeid shrimp Penaeus semisulcatus de Haan. Levels of radiolabeled methionine, total protein and vitellogenin/vitellin (Vg/Vt) were measured in the hemolymph during 24 h following methionine injections. The same parameters were measured 24 h after injection, in the hepatopancreas and ovaries of sacrificed females. Proteins were precipitated by trichloroacetic acid and Vg/Vt was immunoprecipitated by anti-Vt serum. Hemolymphatic protein and Vg/Vt levels were constant throughout the 24 h of the experiment starting from the first sampling of hemolymph, two h after injection. Similar amounts of Vg/Vt were found in the hepatopancreas compared to the ovary 24 h after injection, and 10% of the labeled protein in the ovary and 6.4% in the hepatopancreas were Vg/Vt. Free-labeled methionine was still present in all tissues examined after 24 h. The labeled protein and Vg/Vt in the ovary and the hepatopancreas could not be explained by the hemolymphatic content of the two organs. The hemolymphatic Vg is 24-fold labeled over the ovarian Vt, 24 h after injection. The results indicate more intense involvement of the hepatopancreas in the vitellogenic process than can be deduced from earlier in vitro studies. This study confirms the earlier definition of the oogenic stage of rapid Vt accumulation in the ovary (AOD range of 150-250 {mu}m) and also indicates a role for the hemolymph in transporting Vg between its processing sites.


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Copyright © 1992 by the Marine Biological Laboratory.