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The Biological Bulletin, Vol 184, Issue 3 322-329, Copyright © 1993 by Marine Biological Laboratory

PHYSIOLOGY

The Sequences of Five Neuropeptides Isolated from Limulus using Antisera to FMRFamide

G. Gaus, K. E. Doble, D. A. Price, M. J. Greenberg, T. D. Lee and B. A. Battelle
Institut fur Biologie II (Zoologie), RWTH Aachen, 5100 Aachen, Germany

Five neuropeptides were isolated from CNS extracts of the horseshoe crab Limulus polyphemus by high pressure liquid chromatography (HPLC). The peptides were identified by radioimmunoassays (RIAs) based on two antisera raised to FMRFamide-related peptides (FaRPs). The purified peptides were analyzed by automated sequencing and mass spectrometry, and the following sequences were obtained: DEGHKMLYFamide, GHSLLHFamide, PDHHMMYFamide, DHGNMLYFamide, and GGRSPSLRLRFamide. The first four peptides are members of a novel family with virtually no relationship to FMRFamide. GGRSPSLRLRFamide, on the basis of structural similarity, becomes the second member of a class of FaRPs known previously only from a peptide isolated from mosquito heads. At least one member of the novel family (GHSLLHFamide) inhibits the isolated heart of Limulus.




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Copyright © 1993 by the Marine Biological Laboratory.