Biol. Bull. Sign up for etocs!
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Saigusa, M.
Right arrow Articles by Iwasaki, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Saigusa, M.
Right arrow Articles by Iwasaki, H.

The Biological Bulletin, Vol 197, Issue 2 174-187, Copyright © 1999 by Marine Biological Laboratory

DEVELOPMENT AND REPRODUCTION

Ovigerous-Hair Stripping Substance (OHSS) in an Estuarine Crab: Purification, Preliminary Characterization, and Appearance of the Activity in the Developing Embryos

M. Saigusa and H. Iwasaki
Department of Biology, Faculty of Science, Okayama University, Tsushima 2-1-1 (General Education Buildings), Okayama 700-8530, Japan

Ovigerous-hair stripping substance (OHSS) is an active factor in crab hatch water (i.e., filtered medium into which zoea larvae have been released). This factor participates in stripping off the egg attachment structures (i.e., egg case, funiculus, and the coat investing ovigerous hairs) that remain attached to the female's ovigerous hairs after larval release. Thus this activity prepares the hairs for the next clutch of embryos. OHSS activity of an estuarine crab, Sesarma haematocheir, eluted as a single peak on molecular-sieve chromatography, but this peak still showed two protein bands at 32 kDa and 30 kDa on SDS-PAGE. The two protein bands stained with a polyclonal antiserum raised to the active fractions from molecular-sieve chromatography. Moreover, antibodies purified from this polyclonal OHSS antiserum also recognized both the 32-kDa and 30-kDa bands. OHSS immunoreactivity and biological activity were associated with the attachment structures that remained connected to the ovigerous hairs after hatching. In developing embryos, both protein bands could be stained immunochemically at least 10 days before hatching. But OHSS biological activity appeared only 3 days before hatching. The immunoreactive protein bands were not observed in the zoea, but OHSS bioreactivity was present, though greatly reduced. The 32-kDa protein, at least, is probably an active OHSS, and the 30-kDa protein band may also be OHSS-related. The OHSS appears to be produced and stored by the developing embryo. Upon hatching, most of the material may be trapped by the remnant structures, and the remainder is released into the ambient water.


This article has been cited by other articles:


Home page
 J. Exp. Biol.Home page
O. Gusev, H. Ikeda, T. Okochi, J. M. Lee, M. Hatakeyama, C. Kobayashi, K. Agata, H. Yamada, and M. Saigusa
Purification and cDNA cloning of the ovigerous-hair stripping substance (OHSS) contained in the hatch water of an estuarine crab Sesarma haematocheir
J. Exp. Biol., February 1, 2004; 207(4): 621 - 632.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Bull.Home page
M. Saigusa, M. Terajima, and M. Yamamoto
Structure, Formation, Mechanical Properties, and Disposal of the Embryo Attachment System of an Estuarine Crab, Sesarma haematocheir
Biol. Bull., December 1, 2002; 203(3): 289 - 306.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1999 by the Marine Biological Laboratory.