Biol. Bull.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text Free
Right arrow Full Text (PDF) Free
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via Web of Science (5)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Walker, A.
Right arrow Articles by Lee, R. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Walker, A.
Right arrow Articles by Lee, R. F.
Related Collections
Right arrow Development
Right arrow Crustaceans
Biol. Bull. 204: 50-56. (February 2003)
© 2003 Marine Biological Laboratory

Synthesis of a High-Density Lipoprotein in the Developing Blue Crab (Callinectes sapidus)

Anna Walker1, Seichi Ando2 and Richard F. Lee3,*

1 Department of Pathology, Mercer University School of Medicine, Macon, Georgia 31207
2 Lipoprotein Research Laboratory, Department of Fisheries Science, Kagoshima University, 4-50-20 Shimoarata, Kagoshima 890-0056, Japan
3 Skidaway Institute of Oceanography, Savannah, Georgia 31411

* To whom correspondence should be addressed. E-mail: dick{at}skio.peachnet.edu

An important lipoprotein in the hemolymph of crustaceans is LpI. It transports lipid to peripheral tissues and also has a role in crustacean immune recognition. We employed a monoclonal antibody specific for the LpI peptide to demonstrate by ELISA, western blot and immunohistochemistry the appearance of LpI during development of Callinectes sapidus, the blue crab. LpI was first found in stage 5 embryos and appeared to be synthesized by lateral basophilic cuboidal cells that demonstrated cytoplasmic immunoreactivity for LpI at their interface with the yolk mass. The embryonic cuboidal cells bore a strong cytologic resemblance to the hepatopancreas cells of later stages (zoea, megalopae, adults), which were also immunoreactive for LpI.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2003 by the Marine Biological Laboratory.