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Biol. Bull. 204: 50-56. (February 2003)
© 2003 Marine Biological Laboratory

Synthesis of a High-Density Lipoprotein in the Developing Blue Crab (Callinectes sapidus)

Anna Walker1, Seichi Ando2 and Richard F. Lee3,*

1 Department of Pathology, Mercer University School of Medicine, Macon, Georgia 31207
2 Lipoprotein Research Laboratory, Department of Fisheries Science, Kagoshima University, 4-50-20 Shimoarata, Kagoshima 890-0056, Japan
3 Skidaway Institute of Oceanography, Savannah, Georgia 31411

* To whom correspondence should be addressed. E-mail: dick{at}skio.peachnet.edu

An important lipoprotein in the hemolymph of crustaceans is LpI. It transports lipid to peripheral tissues and also has a role in crustacean immune recognition. We employed a monoclonal antibody specific for the LpI peptide to demonstrate by ELISA, western blot and immunohistochemistry the appearance of LpI during development of Callinectes sapidus, the blue crab. LpI was first found in stage 5 embryos and appeared to be synthesized by lateral basophilic cuboidal cells that demonstrated cytoplasmic immunoreactivity for LpI at their interface with the yolk mass. The embryonic cuboidal cells bore a strong cytologic resemblance to the hepatopancreas cells of later stages (zoea, megalopae, adults), which were also immunoreactive for LpI.






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