HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (1) Citing Articles via Google Scholar Google Scholar Articles by Scholnick, D. A. Articles by Burnett, L. E. Search for Related Content PubMed PubMed Citation Articles by Scholnick, D. A. Articles by Burnett, L. E.

Impact of Exposure to Bacteria on Metabolism in the Penaeid Shrimp Litopenaeus vannamei

¹ Marine Science, Eckerd College, St. Petersburg, Florida 33711
² Grice Marine Laboratory, 205 Fort Johnson, College of Charleston, Charleston, South Carolina 29412

^* To whom correspondence should be addressed, at Dept. of Biology, Pacific University, 2043 College Way, Forest Grove, OR 97116. David.Scholnick{at}pacificu.edu

We hypothesized that aggregation of bacteria and hemocytes at the gill, which occurs as part of the shrimp’s antibacterial immune defenses, would impair normal respiratory function and thereby disrupt aerobic metabolism. Changes in oxygen uptake and lactate accumulation were determined in Litopenaeus vannamei, the Pacific white shrimp, following injection with either saline (control) or a strain of the gram-negative bacterium Vibrio campbellii that is pathogenic in crustaceans. The rate of oxygen uptake was determined during the first 4 h after injection and after 24 h. Injection of bacteria decreased oxygen uptake by 27% (from 11.0 to 8.2 µmol g^–1 h^–1) after 4 h, while saline-injected shrimp showed no change. Decreased oxygen uptake persisted 24 h after Vibrio injection. In well-aerated water, resting whole-animal lactic acid levels increased in shrimp injected with bacteria (mean = 2.59 µmol lactate g^–1 ±0.39 SEM, n = 8) compared to saline-injected control shrimp, but this difference did not persist at 24 h. Exposure to hypercapnic hypoxia (Pco₂ = 1.8 kPa, Po₂ = 6.7 kPa) also resulted in significant whole-body lactic acid differences (mean = 3.99 and 1.8 µmol g^–1 tissue in Vibrio and saline-injected shrimp, respectively). Our results support the hypothesis that the crustacean immune response against invading bacteria impairs normal metabolic function, resulting in depression of oxygen uptake and slightly increased anaerobic metabolism.

This article has been cited by other articles:

				L. E. Burnett, J. D. Holman, D. D. Jorgensen, J. L. Ikerd, and K. G. Burnett Immune Defense Reduces Respiratory Fitness in Callinectes sapidus, the Atlantic Blue Crab. Biol. Bull., August 1, 2006; 211(1): 50 - 57. [Abstract] [Full Text] [PDF]