Biol. Bull.
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Biol. Bull. 216: 1-6. (February 2009)
© 2009 Marine Biological Laboratory

Oral Administration of Pharmacologically Active Substances to Squid: A Methodological Description

William Berk1, Jake Teperman1, Kerry D. Walton1,2, Kazunari Hirata2, Mutsuyuki Sugimori1,2 and Rodolfo R. Llinas1,2,*

1 Marine Biological Laboratory, 7 MBL Street, Woods Hole, Massachusetts 02543
2 Department of Physiology and Neuroscience, NYU School of Medicine, New York, New York 10016

* To whom correspondence should be addressed. E-mail: rodolfo.llinas{at}med.nyu.edu

Abstract. The squid giant synapse is a well-defined experimental preparation for the study of ligand-dependant synaptic transmission. Its large size gives direct experimental access to both presynaptic and postsynaptic junctional elements, allowing direct optical, biophysical, and electrophysiological analysis of depolarization-release coupling. However, this important model has not been utilized in pharmacological studies, other than those implementable acutely in the in vitro condition. A method is presented for oral administration of bioactive substances to living squid. Electrophysiological characterization and direct determination of drug absorption into the nervous system demonstrate the administration method described here to be appropriate for pharmacological research.

Abbreviations: HPLC, high performance liquid chromatography • MPP+, 1-methyl-4-phenylpyridinium






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