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Biol Bull 45: 105-112. (August 1923)
© 1923 Marine Biological Laboratory
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THE EFFECT OF TEMPERATURE, FOOD, AND THE AGE OF THE CULTURE ON THE ENCYSTMENT OF DIDINIUM NASUTUM

S. O. MAST 1 and YASUSHI IBARA 1

1 FROM THE ZOÖLOGICAL LABORATORY OF THE JOHNS HOPKINS UNIVERSITY

1. Didinia encyst most readily at a temperature of 25°-30°, which is also the optimum temperature for growth and fission.

2. They do not encyst in temperature so low or so high that it is injurious. They do not encyst at all below 16° and rarely above 39°.

3. They encyst more freely in cultures supplied with food than in those without food, but this is probably due to greater increase in numbers, resulting in greater accumulation of waste material in the one than in the other.

4. They encyst most readily in culture media, which are probably most favorable for growth and reproduction of the paramecia on which they feed.

5. Encystment serves as protection against unfavorable conditions in reference to food and temperature, but such conditions do not facilitate encystment.

6. Encystment is probably induced by the accumulation of excretory waste material.






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