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1 DEPARTMENT OF ZOÖLOGY, UNIVERSITY OF PENNSYLVANIA
1. Anguillula aceti is highly resistant to acetic acid. To see what is the case in other free-living nematodes Rhabditis elegans was used, and it was found that Rhabditis is much more resistant not only to various kinds of acids, but to other toxic substances also, as compared with the tadpole, Daphnia, Aelosoma and Paramecium.
2. When animals were stained in neutral red and in methylene blue, no case in which the stain entered through the cuticle was observed.
3. The entrance of NaOH and alcohol through the cuticle also could not be observed.
4. The stains, NaOH and alcohol entered through the mouth, and when the anus and vulva were opened, entered through them as well.
5. In the case of worms whose posterior ends had been cut off these substances in question entered through the cut end, as well as the mouth.
6. The conclusion to be drawn from these results seems to be that the impermeability of the cuticle is responsible for the resistance of Rhabditis.
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