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Biol Bull 58: 104-122. (February 1930)
© 1930 Marine Biological Laboratory
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OSMOTIC PROPERTIES OF THE ERYTHROCYTE

I. INTRODUCTION. A SIMPLE METHOD FOR STUDYING THE RATE OF HEMOLYSIS

M. H. JACOBS 1

1 From the Department of Physiology, University of Pennsylvania, and the Marine Biological Laboratory, Woods Hole, Massachusetts

1. A simple method is described by which it is possible to measure with a very satisfactory degree of accuracy the rate of hemolysis where the time involved exceeds approximately one second. If the duration of the experiment is ten seconds or more, a complete graphic record of the entire process up to an apparent degree of hemolysis of between 80 and 90 per cent may be obtained.

2. The method may also be used for the accurate determination of the relative numbers of erythrocytes in different suspensions and, assuming a satisfactory definition for the expression "percentage of hemolysis," for the rapid estimation of the latter, within the range most useful for experimental purposes, with an error of no more than one or two per cent.




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H. N. Glassman
Hemolytic Activity of Some Nonionic Surface-active Agents
Science, June 23, 1950; 111(2895): 688 - 689.
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