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Biol Bull 58: 238-255. (June 1930)
© 1930 Marine Biological Laboratory
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THE EQUILIBRIUM OF OXYGEN WITH THE HEMOCYANIN OF LIMULUS POLYPHEMUS DETERMINED BY A SPECTROPHOTOMETRIC METHOD

ALFRED C. REDFIELD 1

1 From the Department of Physiology, Harvard Medical School, Boston, and the Marine Biological Laboratory, Woods Hole

A spectrophotometric method for measuring the equilibrium of hemocyanin and oxygen is described.

The oxygen dissociation curves of purified hemocyanin of Limulus in the absence of salts and at various hydrogen ion activities are determined.

It is shown that the equilibrium between oxygen and these hemocyanin solutions is defined, as a first approximation, by the mass law on the assumption that the various oxygen-combining groups react independently of one another in their combination with oxygen.

The value of the equilibrium constant of the oxygenation reaction decreases as the pH value increases from 4.5 to 10.4.






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Copyright © 1930 by the Marine Biological Laboratory.