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Biol Bull 62: 205-211. (April 1932)
© 1932 Marine Biological Laboratory
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STUDIES ON THE CHEMICAL NEEDS OF AMOEligBA PROTEUS: A CULTURE METHOD

WILLIAM F. HAHNERT 1

1 From the Mount Desert Island Biological Laboratory, Maine and the Zoölogical Laboratory of the Johns Hopkins University

1. Amoeligba proteus grows and reproduces for several weeks in a balanced salt solution containing potassium chloride, calcium chloride, calcium phosphate, magnesium tribasic phosphate, and Chilomonas paramecium.

2. They also grow in other solutions, but not so well; e.g., in a solution containing sodium chloride and sodium bicarbonate in addition to the above salts.

3. The results obtained indicate that the presence of sodium is not only unnecessary but actually detrimental while that of magnesium and potassium is favorable if not essential for growth and reproduction in Amoeligba proteus.

4. By observing the effect on fission rate and other physiological processes of omitting various elements or altering their concentration in the solution, it is possible to ascertain the relative importance of the elements to the rhizopod protoplasm and the number and kind and amount necessary for growth.






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Copyright © 1932 by the Marine Biological Laboratory.