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Biol Bull 64: 277-288. (June 1933)
© 1933 Marine Biological Laboratory
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HEMOLYTIC ACTION OF SILVER OCCURRING AS AN IMPURITY IN CHEMICALLY PURE SODIUM CHLORIDE

ERIC G. BALL 1

1 From the Marine Biological Laboratory, Woods Hole, Mass., and Department of Physiological Chemistry, Johns Hopkins Medical School

1. The hemolysis of fish erythrocytes by isotonic solutions made from certain brands of sodium chloride has been shown with the aid of the spectrograph to be due to silver occurring as an impurity in those salts.

2. The amount of silver present in such salts is of the order of magnitude of 10-3 to 10-4 per cent and originates from the use of silverlined vessels in the purification process.

3. A simple procedure for the removal of silver from such salts by adsorption on charcoal has been described.

4. Palladium is the only other element that has been found to be as effective as silver in causing hemolysis.

5. Since the toxic action of silver is not limited to the hemolysis of fish erythrocytes, it is suggested that silver-free sodium chloride be used in the preparation of physiological saline solutions.






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Copyright © 1933 by the Marine Biological Laboratory.