EXPERIMENTAL PROCEDURES IN A STUDY OF THE LOCATION AND CONCENTRATION WITHIN THE HOST CELL OF THE VIRUS OF TOBACCO MOSAIC

¹ From the Botanical Laboratory, University of Wisconsin

In this paper experimental evidence is offered which strengthens the view that the virus of typical tobacco mosaic occurs primarily, if not solely, in the protoplasmic components of the cell, rather than in the vacuole. From the observations made it would seem clear that the occurrence of intracellular bodies, in hair cells at least, is coincident with or an accompaniment of relatively high virus concentration. The inclusion bodies, both vacuolate and striated types, are fragile structures disintegrating on contact with the micro-needle or pipette.