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Biol Bull 68: 363-377. (June 1935)
© 1935 Marine Biological Laboratory
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THE VALIDITY OF THE CENTRIFUGE METHOD FOR ESTIMATING AGGREGATE CELL VOLUME IN SUSPENSIONS OF THE EGG OF THE SEA-URCHIN, ARBACIA PUNCTULATA

HERBERT SHAPIRO 1

1 From the Physiological Laboratory, Princeton University, and the Marine Biological Laboratory, Woods Hole, Massachusetts

Observations of the aggregate volume of Arbacia eggs, when centrifuged in capillary tubes, as a function of centrifugal force, and of duration of centrifuging were made. Data on the comparisons of such total cell volume contained in suspensions of Arbacia eggs as evaluated by three methods (counting in hemacytometers, centrifuging at 2,700 and 7,700 x gravity, and direct counts of diluted suspensions) are given. It is submitted that the centrifuge method is reliable, to within approximately 10 per cent, for estimation of the total volume of cells in a suspension of unfertilized eggs of Arbacia punctulata in sea water provided that it be used with the necessary force and duration for sufficient packing of the cells.

Cells so centrifuged are in the living state, and remain viable, for upon removal from the capillary, they will proceed to cleave and to undergo considerable embryological development.






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Copyright © 1935 by the Marine Biological Laboratory.