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Biol Bull 71: 498-547. (December 1936)
© 1936 Marine Biological Laboratory
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THE MOLECULAR WEIGHTS AND PH-STABILITY REGIONS OF THE HEMOCYANINS

INGA-BRITTA 1, ERIKSSON-QUENSEL 1, and SVEDBERG 1

1 From the Institute of Physical Chemistry, The University, Upsala, Sweden

1. Blood from twenty-two species containing hemocyanin as respiratory pigment has been subjected to a detailed ultracentrifugal study.

2. Sedimentation constants have been determined throughout the pH-stability ranges.

3. In some cases sedimentation equilibrium measurements have been carried out. From these data molecular weight values were obtained and correlated with the sedimentation constants.

4. The various hemocyanins are perfectly homogeneous with regard to molecular weight both at the isoelectric point and in the regions where reversible dissociation or association occurs.

5. The stability range for the components of highest molecular weight always comprises the isoelectric point.

6. Except at extremely low and high pH values the dissociation reactions are reversible.

7. The molecular weights of the different hemocyanins and their reversible dissociation products show a relationship of simple multiples.

8. Species belonging to the same order have similar pH-stability diagrams. Each species has a characteristic diagram by means of which it can be identified. In doubtful cases the isoelectric point may be used as complementary evidence.




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