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Biol Bull 80: 69-78. (February 1941)
© 1941 Marine Biological Laboratory
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THE EFFECT OF SAPONIN ON THE OSMOTIC REMOLYSIS OF CHICKEN ERYTHROCYTES

F. R. HUNTER 1, S. B. BARBER 1, and A. P. CAPUTI 1

1 From the Zoölogy Department, Rhode Island State College, Kingston, Rhode Island

1. Chicken erythrocytes exposed to low concentrations of saponin have their membranes altered.

2. By removing the saponin and resuspending the cells in Ringer-Locke, they will remain unhemolyzed for several days, even though the membranes have been altered.

3. These cells are more permeable to both lipoid-soluble and lipoid-insoluble molecules.

4. The penetration of both types of molecules appears to be affected equally.

5. The rate of penetration of a large molecule such as malonamide is increased more by this treatment than the rate of penetration of a smaller molecule such as glycerol.

6. The fragility of these cells is not increased by this treatment.






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Copyright © 1941 by the Marine Biological Laboratory.